A high-throughput screening method based on the Mizoroki-Heck reaction for isolating itaconic acid-producing fungi from soils
نویسندگان
چکیده
منابع مشابه
Mechanisms of the Mizoroki–Heck Reaction
The palladium-catalysed Mizoroki–Heck reaction is the most efficient route for the vinylation of aryl/vinyl halides or triflates. This reaction, in which a C C bond is formed, proceeds in the presence of a base (Scheme 1.1) [1, 2]. Nonconjugated alkenes are formed in reactions involving cyclic alkenes (Scheme 1.2) [1e, 2a,c,e,g] or in intramolecular reactions (Scheme 1.3) [2b,d–g] with creation...
متن کاملIsolation and Screening of Xylanase Producing Fungi from Forest Soils
Enzymes are distinct natural biological polymers which can catalyze the chemical/ biological reactions and convert substrates to specific product (Haq et al. 2006). Xylanase [E.C.3.2.1.8] are a class of hydrolytic enzymes which can hydrolyze the straight polysaccharide -1, 4-xylan in hemicelluloses which is a noteworthy component of secondary cell wall of plants (Dhiman et al. 2008). Xylan, the...
متن کاملLabeling of alprenolol with fluorescent aryl iodide as a reagent based on Mizoroki-Heck coupling reaction.
A novel fluorescent labeling method for alprenolol was developed based on Mizoroki-Heck coupling reaction. We designed and synthesized fluorescent aryl iodide, 4-(4,5-diphenyl-1H-imidazol-2-yl)iodobenzene (DIBI) as a labeling reagent. DIBI has a lophine skeleton carrying an iodide atom acting as fluorophore and reactive center, respectively. In order to evaluate the usefulness of DIBI, a high-p...
متن کاملHigh throughput automated colorimetric method for the screening of l-lactic acid producing microorganisms
Lactic acid is a valuable and fully degradable organic acid with promising applications in poly-lactic acid production (Taskila S and Ojamo, 2013 [1]). Despite their efficiency, the cost of the current lactic acid bio-processes is still an obstacle to this application (Miller et al., 2011 [2]). To ameliorate lactic acid producing strains, researchers are using mutations and metabolic engineerin...
متن کاملIsolation of eicosapentaenoic acid-producing fungi from soil based on polymerase chain reaction amplification.
A method was developed for rapid screening and isolation of eicosapentaenoic acid (EPA)-producing soil fungi through polymerase chain reaction (PCR) amplification. Genes coding for delta6 fatty acid desaturase and delta5 fatty acid desaturase were used as molecular markers for screening these EPA-producing fungi from soil. Three out of 65 soil fungi gave positive results through PCR amplificati...
متن کاملذخیره در منابع من
با ذخیره ی این منبع در منابع من، دسترسی به آن را برای استفاده های بعدی آسان تر کنید
ژورنال
عنوان ژورنال: Heliyon
سال: 2019
ISSN: 2405-8440
DOI: 10.1016/j.heliyon.2019.e02048